真核生物細胞を改変する方法
- 专利权人:
- 发明人:
- 申请号：
- JP20120032592
- 公开号：
- JP5692863(B2)
- 申请日:
- 2012.02.17
- 申请国别(地区):
- 日本
- 年份:
- 2015
- 代理人:
- 摘要：
A method for engineering and utilizing large DNA vectors to target, via homologous recombination, and modify, in any desirable fashion, endogenous genes and chromosomal loci in eukaryotic cells. These large DNA targeting vectors for eukaryotic cells, termed LTVECs, are derived from fragments of cloned genomic DNA larger than those typically used by other approaches intended to perform homologous targeting in eukaryotic cells. Also provided is a rapid and convenient method of detecting eukaryotic cells in which the LTVEC has correctly targeted and modified the desired endogenous gene(s) or chromosomal locus (loci) as well as the use of these cells to generate organisms bearing the genetic modification.
- 来源网站：
- 中国工程科技知识中心
