An optical sectioning apparatus combining Mirau optical interference microscopy and fluorescence microscopy, including: a wide band light source ； an optical circulator, facing the wide band light source for splitting an incident light beam into a reflected light beam and a transmitted light beam； a short wavelength light source ； a first dichroic splitter, facing the short wavelength light source and the optical circulator respectively, and being capable of providing a light-blocking effect on a band of wavelengths shorter than a preset wavelength； a Mirau interference objective lens, having a collimated side facing the first dichroic splitter； a sample carrier unit facing a focal side of the Mirau interference objective lens； a projection lens, having a light entrance side facing the optical circulator； and a sensor unit facing a light exit side of the projection lens.一種整合米洛(Mirau)光學干涉顯微術與螢光顯微術之光學切層裝置，其具有：一寬頻光源裝置，用以產生一寬頻光束；一光環行器，具有一第一側邊、一第二側邊及一第三側邊，所述第一側邊係面對該寬頻光源裝置以將一入射光束分成一透射光束及由所述第二側邊穿出之一反射光束；一短波光源裝置，用以產生一短波光束；一第一二色分光器，具有一第一側邊、一第二側邊及一第三側邊，所述第一側邊係面對該短波光源裝置，該第一二色分光器係用以使波長短於一預設波長之光束無法穿透，且該短波光束之波長小於該預設波長；一米洛干涉物鏡，具有一平行光側及一聚光側，該平行光側係面對該第一二色分光器之所述第二側邊；一樣本承載單元，面對該米洛干涉物鏡之聚光側且係用以承載一染有螢光劑之樣本；一投影透鏡，具有一入光側及一出光側，該入光側係面對該光環行器之所述第三側邊；以及一感測單元，係面對該投影透鏡之所述出光側。
