The use of microorganisms to make omega- 1-functionalized chemicals and fuels, (e.g. omega- 1-functionalized carboxylic acids, alcohols, hydrocarbons, amines, and their beta- functionalized derivatives and lactams and lactones), by utilizing an iterative carbon chain elongation pathway that uses omega- 1-functionalized primer units. The core enzymes in the pathway include thiolase, dehydrogenase, dehydratase and reductase. Native or engineered thiolases catalyze the condensation of omega- 1-functionalized acyl-CoA primers with acetyl- CoA as the extender unit to generate omega- 1-functionalized β-keto acyl-CoA. Dehydrogenase converts omega- 1-functionalized β-keto acyl-CoA to omega- 1-functionalized β-hydroxy acyl-CoA. Dehydratase converts omega- 1-functionalized β-hydroxy acyl-CoA to omega- 1-functionalized enoyl-CoA. Reductase converts omega- 1-functionalized enoyl-CoA to omega- 1-functionalized acyl-CoA. The platform can be operated in an iterative manner (i.e. multiple turns) by using the resulting omega- 1-functionalized acyl-CoA as primer and acetyl-CoA as the extender unit in subsequent turns of the cycle. Termination pathways acting on any of the four omega- 1-functionalized CoA thioester intermediates terminate the platform and generate various omega- 1-functionalized carboxylic acids, alcohols, hydrocarbons, and amines with different degrees of β-reduction and lactams and lactones.Linvention concerne lutilisation de micro-organismes dans la production de produits chimiques et de combustibles fonctionnalisés en oméga-1 (par exemple des acides carboxyliques, des alcools, des hydrocarbures, des amines fonctionnalisés en oméga-1, et leurs dérivés bêta-fonctionnalisés et des lactames et des lactones), en faisant appel à une voie délongation itérative de la chaîne carbonée qui utilise des motifs damorces fonctionnalisées en oméga-1. Les enzymes centrales dans ladite voie comprennent des thiolase, déshydrogénase, déshydratase et réductase. Les thiolases manipulé
