The gene introduction medicine which is superior in safety and introduction efficiency, the kit for gene introduction and that gene introduction method is offered are designated as topic.The below-mentioned formula being the gene introduction medicine composition which includes the chemical compound where is displayed with either DL G1 - DL G4DL G1: R1 R2 NX (XH2) 2DL G2: R1 R2 NX (X (XH2) 2) 2DL G3: R1 R2 NX (X (X (XH2) 2) 2) 2DL G4: R1 R2 NX (X (X (X (XH2) 2) 2) 2) 2(X shows ch2ch2conhch2ch2n .),The inside R of the above-mentioned formula1 is the unsaturated long chain aliphatic basis, the gene introduction medicine composition which features that at the same time R2 is the unsaturated long chain aliphatic basis or the saturation long chain aliphatic basis, is designated as the solution expedient.安全性及び導入効率に優れた遺伝子導入剤、遺伝子導入用キット及び遺伝子導入方法を提供することを課題とする。下記式DL-G1~DL-G4のいずれかで表される化合物を含む遺伝子導入剤組成物であってDL-G1:R1R2NX(XH2)2DL-G2:R1R2NX(X(XH2)2)2DL-G3:R1R2NX(X(X(XH2)2)2)2DL-G4:R1R2NX(X(X(X(XH2)2)2)2)2(Xは、-CH2CH2CONHCH2CH2N-を示す。)、上記式中R1が不飽和長鎖脂肪族基であり、かつR2が不飽和長鎖脂肪族基又は飽和長鎖脂肪族基であることを特徴とする遺伝子導入剤組成物を、その解決手段とする。