Disclosed herein is an eco-friendly, non-aqueous and stable antimicrobial solution composition comprising (a) about 0.1 to 20.0 wt % of a Hinokitiol； and (b) about 80.0 to 99.9 wt % of bio-derived 1,3-propanediol and/or bio-derived sorbitan caprylate. Also disclosed is a process for preparing and method of using the composition. In accordance with another aspect of the present application, the eco-friendly, nonaqueous antimicrobial composition is capable of inhibiting or killing gram (+) bacteria, gram (−) bacteria, yeasts and/or fungi.
