DE FREES, SHAWN,ZOPF, DAVID,BAYER, ROBERT,BOWE, CARYN,HAKES, DAVID,CHEN, XI
申请号：
ES10012794
公开号：
ES2538342T3
申请日:
2002.10.09
申请国别(地区):
ES
年份:
2015
代理人:
摘要：
An in vitro procedure, without conjugate formation cells between a follicle stimulating hormone (FSH) peptide and a water soluble polymer, in which the water soluble polymer covalently binds to the FSH peptide through a intact glycosyl binding group, said intact glycosyl binding group being interposed between and covalently linked to both the peptide and the water soluble polymer, wherein the water soluble polymer is not a naturally occurring sugar and is selected from the group consisting of polyalkylene oxides and polypeptides, the FSH peptide comprising a glycosyl moiety having the formula: ** Formula ** in which a, b, c, d, i, q, r, s, tyu are members independently selected from 0 and 1； e, f, g and h are independently selected members of the whole numbers between 0 and 6； j, k, l and m are independently selected members of the whole numbers between 0 and 100； v, w, x e y are 0； and R is a modifying group, a mannose or an oligomanose, said method comprising: (a) contacting the FSH peptide with a glycosyltransferase and a modified glycosyl donor, comprising a glycosyl moiety that is a substrate for glycosyltransferase covalently bound to the water soluble polymer, under conditions suitable for the formation of the intact glycosyl binding group.Un procedimiento in vitro, sin células de formación de un conjugado entre un péptido de la hormona estimuladora del folículo (FSH) y un polímero soluble en agua, en el que el polímero soluble en agua se une covalentemente al péptido de FSH a través de un grupo de unión a glicosilo intacto, estando dicho grupo de unión a glicosilo intacto interpuesto entre y unido covalentemente tanto al péptido como al polímero soluble en agua, en el que el polímero soluble en agua no es un azúcar de origen natural y está seleccionado del grupo que consiste en óxidos de polialquileno y polipéptidos, comprendiendo el péptido de FSH un resto de glicosilo que tiene la fórmula:**Fórmula** en la que a, b, c, d, i, q, r, s, t y u son miembros ind
