３段階方式矩形波多重パルスを利用した藻類細胞への遺伝子導入法
- 专利权人:
- 发明人:
- 申请号：
- JP20130138581
- 公开号：
- JP5721191(B2)
- 申请日:
- 2013.07.02
- 申请国别(地区):
- 日本
- 年份:
- 2015
- 代理人:
- 摘要：
To provide a technology that solves the above-mentioned problems, and is directly applicable to eukaryotic algal cells with cell-wall, the technology enabling gene transfer and transformation to be performed with high efficiency and good reproducibility irrespective of species of algae. Also provided is a method of transferring an exogenous gene into a eukaryotic algal cell, the method comprising performing electroporation using multiple square-wave pulses to the solution containing a cell of a green alga with cell-wall, and a nucleic acid molecule by the following three steps: applying a square-wave electric pulse (first electric pulse) with a high voltage for a short period of time under the condition that its total electric energy falls within a predetermined range; then applying a square-wave electric pulse (second electric pulse) with a low voltage for a long period of time two or more times; and then applying a square-wave electric pulse (third electric pulse) that is opposite in polarity to the seco
- 来源网站：
- 中国工程科技知识中心
