CORNELL UNIVERSITY;ACADEMISCH MEDISCH CENTRUM;THE SCRIPPS RESEARCH INSTITUTE
发明人:
DE LA PEÑA, Alba Torrents,SANDERS, Rogier,WARD, Andrew B.,WILSON, Ian Andrew,JULIEN, Jean-Philippe,MOORE, John P.
申请号：
EPEP2016/073377
公开号：
WO2017/055522A1
申请日:
2016.09.29
申请国别(地区):
EP
年份:
2017
代理人:
摘要：
The invention relates to an isolated, recombinant or synthetic polypeptide comprising an Env polypeptide of an HIV virus comprising a gp120 polypeptide portion and at least a gp41 ectodomain polypeptide portion from HIV-1 or a gp125 polypeptide portion and at least a gp36 ectodomain polypeptide portion from HIV-2, wherein said gp120 or gp125 polypeptide portion and said gp41 or gp36 ectodomain polypeptide portion comprise a first cysteine residue at an amino acid position equivalent to amino acid position 49, 50, 51, 71, 72 or 73 and a second cysteine residue at an amino acid position equivalent to amino acid position of a residue selected from the group consisting of: 553, 554, 555, 556, 557, 558, 559, 560, 561, 562, 563, 564, 565, 566, 567, 568, 569 and 570, wherein numbering is according to the HxB2 reference sequence having the amino acid sequence of SEQ ID NO: 1, and wherein said first and said second cysteine residues form an intermolecular disulfide bond. The invention further relates to an isolated, recombinant or synthetic polypeptide comprising an Env polypeptide of an HIV virus comprising a gp120 polypeptide portion and at least a gp41 ectodomain polypeptide portion from HIV-1 or a gp 125 polypeptide portion and at least a gp36 ectodomain polypeptide portion from HIV-2, wherein said Env polypeptide comprises at least one amino acid residue selected from the group consisting of: 64K and 66R； and 302F, 302W, 304F, 304W, 306L, 307F, 307W, 308L, 315Y, 315K, 3151, 315W, 315F, 315V, 316Y, 316K, 3161, 316F, 316W, and 316V； and 555K or 556K； and 137A, wherein numbering of said at least one amino acid residue is according to the HxB2 reference sequence having the amino acid sequence of SEQ ID NO: 1, and wherein said at least one amino acid residue is at an amino acid position equivalent to the amino acid position of SEQ ID NO: 1.L'invention concerne un polypeptide isolé, recombinant ou synthétique comprenant un polypeptide Env d'un virus du VIH comprenant une part
