PRUSSAK, CHARLES E.,KIPPS, THOMAS J.,CANTWELL, MARK J.
申请号：
CA2486918
公开号：
CA2486918C
申请日:
2003.05.23
申请国别(地区):
CA
年份:
2013
代理人:
摘要：
The present invention provides for an isolated polynucleotide sequenceencoding a chimeric CD154, comprising a first nucleotide sequence encoding anextracellular subdomain of non-human CD154, preferably murine CD154, thatreplaces a cleavage site of human CD154, and a second nucleotide sequenceencoding an extracellular subdomain of human CD154 that binds to a human CD154receptor. The first nucleotide sequence preferably further encodes anextracellular subdomain of non~human CD154 that is critical for expression ofsaid CD154 by cells, such as human CD40+ cells, and another extracellulardomain that detects expression of the chimeric CD154 by binding to anti-murineantibodies. The second nucleotide sequence preferably further encodes anextracellular subdomain of human CD154 to which functionally inhibitory anti-CD154 antibodies bind. The present invention also provides for the chimericCD154 that is encoded by the above-described polynucleotide sequence, anexpression vector and a genetic vector comprising the polynucleotide sequence,a host cell comprising the expression vector or the genetic vector, a processfor producing the chimeric CD154, and methods for utilizing the expressionvectors and genetic constructs containing the chimeric CD154 polynucleotidesequences.
