This invention provides a preparation method of a scratch-resistant and antibacterial film. The preparation method comprises the steps: preparing a film layer mixing nanometer metal particles and a photopolymer to form a coating coating the film layer with the coating to form a scratch-resistant and antibacterial layer on the film layer and drying the scratch-resistant and antibacterial layer on the film layer, and carrying out irradiation by using an UV lamp and curing to prepare the scratch-resistant and antibacterial film. The scratch-resistant and antibacterial film prepared by using the preparation method provided by this invention has the effect of improving the scratch resistance and antibacterial property, and also solving the problem of reducing the antibacterial effect caused by insufficient anti-scratch strength in the prior art.本發明提供一種耐刮抗菌膜之製備方法,其步驟包含齊備一薄膜層;將奈米金屬粒子以及光聚合物混合為一塗料;將該塗料塗佈於薄膜層,以於薄膜層上形成一耐刮抗菌層;以及,烘乾薄膜層上之耐刮抗菌層,並以UV燈照射固化,以製得一耐刮抗菌膜。藉由本發明之製備方法所製得之耐刮抗菌膜具有增加耐刮及抗菌效果,解決現有技術之抗菌膜之抗刮強度不足而降低抗菌時效之問題。S1‧‧‧步驟1S2‧‧‧步驟2S3‧‧‧步驟3S4‧‧‧步驟4