A method and device for isolating tissue cells from a liquid is provided for integrating a mixture of the tissue cells and the liquid into a vacuum stream and isolating again from the vacuum stream in a tissue collection container (13). The tissue cells are filtered out of the mixture by means of gravity and the remaining liquid is re-integrated into the vacuum stream. In order to further develop said method and device to improve the care of the tissue cells and to reduce the filtration time, the vacuum stream that is isolated from the mixture inside the tissue collection container (13) is temporarily interrupted in order to generate a pressure that also acts on the mixture. In terms of the device, the connection between the lower collection chamber (23) for the liquid and the upper vacuum chamber (25) is formed by a blockable bypass line (27).
