The present invention relates to the methods for preparing aerosol with what. More specifically, the present invention provides the method for the aerosol with what preparation immunoglobulin (Ig) list variable domains, wherein the significant amount for reducing aggregation and being formed. Invention additionally provides aerosols prepared by the method for the present invention, and with the composition in what method of the invention. The invention additionally relates to the methods for preparing the composition with what, container, kit and aerosol delivery systems and their purposes comprising the composition.本發明涉及用於製備氣霧劑的方法。更具體地,本發明提供了用於製備免疫球蛋白單可變結構域的氣霧劑的方法,其中顯著減少聚集體形成的量。本發明另外提供了通過本發明的方法製備的氣霧劑,以及用於本發明的方法中的組合物。本發明另外涉及用於製備所述組合物的方法,包含所述組合物的容器、試劑盒和氣霧劑遞送系統,以及它們的用途。