To improve a holding power of a first seedling picking claw and a second seedling picking claw to the seedlings and prevent complication of a mounting structure.SOLUTION: A seedling pickup device includes: a first claw mounting part and a second claw mounting part reciprocating to the tray receiving the seedlings; a first seedling picking claw supported to the first claw mounting part; a second seedling picking claw supported to the second claw mounting part; a biasing member for biasing a tip interval between the first seedling picking claw and the second seedling picking claw in the direction to be widened; and a pressing member for pressing the first claw mounting part and the second claw mounting part to narrow the tip interval when the first seedling picking claw and the second seedling picking claw move toward the tray. The first seedling picking claw has a first main claw mounted to the first claw mounting part, and a first secondary claw comprising a first rising part rising up from the first main claw and a first secondary rod part extending toward the tip side of the first main claw from the first rising part. The second seedling picking claw has a second main claw mounted to the second claw mounting part, and a second secondary claw comprising a second rising part rising up from the second main claw and a second secondary rod part extending toward the tip side of the second main claw from the second rising part.SELECTED DRAWING: Figure 1【課題】第1苗取爪及び第2苗取爪の苗に対する保持力を向上させると共に取付構造の複雑化を防止する。【解決手段】苗を収容したトレイに対して進退する第1爪取付部及び第2爪取付部と、第1爪取付部に支持された第1苗取爪と、第2爪取付部に支持された第2苗取爪と、第1苗取爪と第2苗取爪との先端間隔を拡げる方向に付勢する付勢部材と、第1苗取爪及び第2苗取爪がトレイに向けて移動する際に、第1爪取付部及び第2爪取付部を押圧して先端間隔を縮める押圧部材と、を備え、第1苗取爪は、第1爪取付部に取り付けられた第1主爪と、第1主爪から立ち上がる第1立上がり部及び第1立上がり部から第1主爪の先端側に向けて延びる第1副杆部を含む第1副爪とを有し、第2苗取爪は、第2爪取付部に取り付けられた第2主爪と、第2主爪から立ち上がる第2立上がり部及び第2立上がり部から第2主爪の先端側に向けて延びる第2副杆部を含む第2副爪とを有する。【選択図】図1