An anti-fatty liver agent containing a cell and/or a culture of the lactic acid bacterium Latobacillus gasseri as an active ingredient is provided. Also disclosed is a pharmaceutical agent having an anti-fatty liver activity or an anti-fatty liver beverage or food containing a cell and/or a culture of the lactic acid bacterium Latobacillus gasseri as an active ingredient. Methods of treating fatty liver in a subject by administering a cell and/or a culture of the lactic acid bacterium Latobacillus gasseri are also provided.