Provided is a preparation method of a porous hyaluronic acid sponge comprising the steps of: dissolving hyaluronic acid in an aqueous sodium hydroxide solution； adding an epoxy-based crosslinking agent to the resultant aqueous sodium hydroxide solution in which hyaluronic acid is dissolved and homogenizing the hyaluronic acid solution； hydrogelating the homogenized hyaluronic acid solution； washing the hydrogelated hyaluronic acid hydrogel with ultrapure water； swelling the washed hyaluronic acid hydrogel to attain porosity； and freeze-drying the hyaluronic acid hydrogel to obtain a porous hyaluronic acid sponge.
