The present invention relates to a tissue engineering device, which comprises: a first half-tube a second half-tube assembling with the first half-tube to form a hollow tube, wherein a first end of the hollow tube has a first opening, a second end of the hollow tube has a second opening a first cap having a first penetrating hole, and assembling on the first end of the hollow tube to open or close the first opening and a second cap having a second penetrating hole, and assembling on the second end of the hollow tube to open or close the second opening wherein when the tissue engineering device is installed on an observed body, blood vessels of the observed body penetrate through the first penetrating hole of the first cap, the hollow tube, and the second penetrating hole of the second cap serially.