An optical detection method applying for detection of the subject matter β -amyloid in aqueous humor, lens, and retina of human eye or sediment combined by α β -crystallin and β -amyloid in lens is provided, wherein refer to an absorption spectrum of the detection of the subject matter for selection a frequency to emit a light, whose frequency is equal or close to one resonant excitation frequency of electronic molecular energy levels of the detection of the subject matter, to a testing area in human eye so as to excite the detection of the subject matter to generate resonance-enhanced Raman effect or pre-resonance Raman effect to form a detection spectrum. The concentration of the detection of the subject matter could be estimated by one of the peaks intensity of the detection spectrum. The optical detection method is a non-invasive and non-labeled in vitro test, and the detection result could be applied for early detecting related diseases such as Alzheimer disease.一種光學檢測方法,適於檢測人眼的前房液、水晶體或視網膜內的β-澱粉類蛋白(β-amyloid,Aβ)或者水晶體內的αβ晶狀體蛋白(αβ-crystallin)與β-澱粉類蛋白結合後之沉澱物,其中參照所檢測物質的吸收光譜來選擇入射光的頻率入射至人眼內的待測區,使此光線的頻率等同或接近檢測物質分子能階的電子躍遷之共振模態的激發頻率,以激發檢測物質產生共振拉曼效應或類共振拉曼效應,而獲得檢測光譜。依據檢測光譜的波峰強度可以估計物質的濃度。本發明之光學檢測方法為非侵入式且免標定的體外檢測,而檢測結果可被應用於早期偵測相關病症,例如阿茲海默症。100...光學檢測方法110~130...步驟
