Method and apparatus for hand disinfection quality control comprising the steps of providing hand disinfection medium comprising light reflecting particles responsive to light mostly outside the visible spectral range of light, applying the hand disinfection medium comprising the light reflecting particles in a prescribed manner for a prescribed time, illuminating the disinfected hands with a light source providing light in a spectral range for activating the reflecting particles. The method further comprises providing digital images of the hands from both sides, determining the concentration of reflecting particles, comparing determined concentration values with a predetermined threshold value corresponding to acceptable hand cleanness, and determining whether the quality of disinfection is acceptable or not according to the result of comparison. In the method according to the invention for each person individual threshold value is stored, and the concentration value determined during measuring is compared with the individual threshold value.