The present invention discloses a manufacturing method of artificial blood vessel, which includes the following steps: utilizing 3D printing technology to create a template； preparing an active polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane (POSS-PCU) and activating POSS-PCU to form the active POSS-PCU； mixing the active POSS-PCU with stem cells and utilizing 3D printing technology to form an artificial blood vessel； processing with plasma technology. Finally, the template is removed to form the artificial blood vessel having the channel and a three-layered structure. The manufacturing method of the present invention may produce an artificial blood vessel having three-layered structure for delivering nutrition and oxygen, removing metabolic waste, and enhancing blood compatibility and bio-stability, so as to solve the problems in the prior art that only a single-layer artificial blood vessel could be produced and it is easy to occur with the vascular blockage.本發明揭示一種人工血管之製備方法，其步驟包含：利用3D列印技術製備一模板。齊備一活化之多面體矽氧烷寡聚物聚氨酯(POSS-PCU)，並活化POSS-PCU以形成之活化之POSS-PCU。將活化之POSS-PCU以及幹細胞進行混合，並利用3D列印技術形成一人工血管，再以電漿技術處理。最後移除模板，形成一具有通道且為三層結構之人工血管。本發明之製備方法可製得具三層結構之人工血管，其可運送養分、氧氣及移除代謝廢物、增加血液相容性及生物穩定性，藉以解決既有技術僅能製造單層人工血管之問題以及容易發生血管堵塞等問題。
