WAYNE MATTHEW CRISMANI,KEVIN DAY,GREGORY D. MAY,JUSTIN ANDREW SCHARES,YUE YUN
申请号：
US15211053
公开号：
US20180016645A1
申请日:
2016.07.15
申请国别(地区):
US
年份:
2018
代理人:
摘要：
Methods and compositions to genotype microspores and/or pollen grains are provided. Methods provided include using meiotically-related products to non-destructively determine the genotype of one of the meiotically-related products such that it can be further used. For example, methods to obtain a genotype of a microspore are provided. The methods include: isolating tetrads, separating the tetrads to obtain four tetrad microspores； genotyping three of the four tetrad microspores, and inferring the genotype of the fourth tetrad microspore. Methods are also provided for generating doubled haploid plants from the microspores that are selected for further analysis based on inferred genotypes. Viable genotyped pollen produced by these methods, as well as seed, cells, plants, germplasm, progeny, and plant parts derived therefrom are also provided.
