The present invention relates to a pincer for binding to a target material, wherein an antibody or a fragment thereof binding to a first target site of the target material and an aptamer binding to a second target site are conjugated via a linker, a preparation method thereof, a composition for detecting or separating the target material comprising the pincer, a kit for detecting or separating the target material comprising the composition, and a method for detecting or separating the target material using the kit.Also, the present invention relates to a drug carrier comprising the pincer for binding to a target material, wherein an aptamer conjugated to a second target site of the pincer loads the drug.The antibody-aptamer pincer comprising the antibody and the aptamer, which target for the different binding sites on the same material of the present invention shows a ten- to hundred-fold increased binding affinity for the target molecule compared to using the antibody or the aptamer alone, and thus can be usefully applied for detection and/or separation of traces of the target material.Further, the affinity of the drug carrier prepared by the binding of the antibody with the aptamer for the target molecule is increased, and thus, it can be used as a drug carrier against harmful tumors.
