МАКВИРТЕР Джон (US),МАКДОНАЛД Линн (US),МЕРФИ Эндрю Джей. (US)
申请号：
RU2014141537
公开号：
RU2014141537A
申请日:
2013.03.15
申请国别(地区):
RU
年份:
2016
代理人:
摘要：
1. A genetically modified non-human animal comprising in its germline immunoglobulin light chain locus, which contains a single gene sequence of a rearranged variable region of the light chain variable region comprising the sequence of segments of the human immunoglobulin Jcheloveka vulgaris, wherein the sequence segments vulgaris Jcheloveka single rearranged gene sequences of the light human immunoglobulin chains contain at least one histidine codon which is not encoded by the corresponding gene sequences vulgaris Jzarodyshevoy cheloveka.2 line segments. Animal according to Claim. 1, in which only the sequence of a rearranged human immunoglobulin variable region operably linked to the gene sequence of the constant region of the light chain immunoglobulina.3. An animal according to claim. 2, in which the gene sequence of the constant region of an immunoglobulin light chain is different from the gene sequence of human light chain constant region immunoglobulina.4. Animal according to Claim. 3, which differs from the human gene sequence of the constant region of an immunoglobulin light chain is different from the endogenous gene sequence of the human constant region of the light chain immunoglobulina.5. Animal according to Claim. 1 further comprising in its germline immunoglobulin heavy chain locus which contains the gene sequence of unrearranged immunoglobulin variable region heavy chain comprising segments V, D and Jcheloveka, Fu
