Arizona Board of Regents for and on Behalf of Arizona State University
发明人:
Mason, Hugh S.,Huang, Zhong,Chen, Qiang,Arntzen, Charles J.,Yuan, Shuo,Hjelm, Brooke
申请号：
AU2009285667
公开号：
AU2009285667B2
申请日:
2009.08.27
申请国别(地区):
AU
年份:
2015
代理人:
摘要：
Plant viral vectors have great potential in rapid production of proteins, but no simple Here a geminivirus-based system for high-yield and rapid production of oligomeric protein complexes, including virus-like particle (VLP) vaccines and monoclonal antibodies (mAbs) is described. In particular, a single vector that contains two non-competing replicons for transient expression in Nicotiana benthamiana leaves is described. The correct assembly of these subunit proteins into functional oligomeric structures (VLPs or full-size mAb) is also described. This system advances plant transient expression technology by eliminating the need for non-competing viruses, and thus, enhances the realistic commercial application of this technology for producing multiple-subunit protein complexes.
