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Probes for detection and quantification of post-translational modifications of proteins
专利权人:
塩野義製薬株式会社;国立大学法人 東京大学
发明人:
佐藤 守俊,高橋 浩治
申请号:
JP2009247098
公开号:
JP5513844B2
申请日:
2009.10.27
申请国别(地区):
JP
年份:
2014
代理人:
摘要:
<;P>;PROBLEM TO BE SOLVED: To provide a probe for detection-quantification of the posttranslational modification of a protein as a tool useful for nondestructively detecting or quantifying the posttranslational modification, especially phosphorylation, of the protein in a living cell or in the living body of an animal, a plant or the like. <;P>;SOLUTION: There is provided the probe sequentially having the following amino acid sequences (1) to (5) from the N-terminal side: (1) The amino acid sequence of a cap protein; (2) the amino acid sequence of a modifiable polypeptide to whose N-terminal side a ubiquitin ligase-recognizing sequence is connected; (3) a ubiquitinizable linker sequence; (4) a modification recognizable sequence, and (5) the amino acid sequence of a chromogenic protein. <;P>;COPYRIGHT: (C)2011,JPO&INPIT
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