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DIFFERENCIATION EX VIVO DE CELLULES TUEUSES NATURELLES A PARTIR DE CELLULES HEMATOPOIETIQUES CD34+
专利权人:
IPD-THERAPEUTICS B.V.
发明人:
SPANHOLTZ, JAN,DOLSTRA, HARMEN,DOLSTRA, HARRY
申请号:
CA2864283
公开号:
CA2864283A1
申请日:
2013.02.07
申请国别(地区):
CA
年份:
2013
代理人:
摘要:
The present invention relates to the ex vivo differentiation of NK cells from CD34+ hematopoietic stem cells. Such NK cells and their progenitor cells can be used in therapies of a broad range of malignancies. In the present invention it is shown that IL-12 modulates ex vivo NK cell differentiation. Specific, we achieved significantly higher expression of KIR, CD16 and CD62L in the presence of IL-12 in the cell culture system. The induction of receptor expression by IL-12 occurred predominantly on an augmented population of CD33+NKG2A+ NK cells early during NK cell differentiation. These cells further show enhanced cytolytic activity against MHC class I positive AML targets. In line with the enhanced CD16 expression, IL-12 modulated ex vivo generated NK cells exhibit an improved antibody-dependent-cytotoxicity, using anti CD20 antibody on various B cell targets. Additional to the enhanced expression of CD62L, we show that this cell population consists of a specific chemokine receptor profile. By showing an increased capacity for adhesion to lymphendothelial cells and a specific chemokine receptor profile, we show that IL-12 provided the ex vivo generated NK cells with specific tissue-homing abilities.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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