PROBLEM TO BE SOLVED: To make it possible to obtain in a short time an image information showing a biological substance distribution such as an oxygen saturation distribution.SOLUTION: The method comprises: imaging biological tissue using each of one or more light beams containing a first wavelength region where the light absorption increases with the increase of the molar concentration ratio to obtain one or more first imaging data sets imaging the biological tissue using each of one or more light beams containing a second wavelength region where the light absorption decreases with the increase of the molar concentration ratio to obtain one or more second imaging data sets for each pixel, calculating the light absorption of the biological tissue with respect to a light beam used for obtaining each imaging data set, on the basis of each imaging data set for each pixel, calculating an index showing a molar concentration ratio between a first biological substance and a second biological substance, on the basis of the difference between the sum of light absorptions of the biological tissue calculated based on the one or more first imaging data sets and the sum of light absorptions of the biological tissue calculated based on the one or more second imaging data sets and creating a distribution image on the basis of the indexes.SELECTED DRAWING: Figure 1COPYRIGHT: (C)2016,JPO&INPIT【課題】酸素飽和度分布等の生体物質の分布を表わす画像情報を短時間で取得可能にする。【解決手段】モル濃度比の増大と共に光吸収が増大する第1種波長域を含む一種以上の光のそれぞれを用いて生体組織を撮像して一つ以上の第1撮像データを取得するステップと、モル濃度比の増大と共に光吸収が減少する第2種波長域を含む一種以上の光のそれぞれを用いて生体組織を撮像して一つ以上の第2撮像データを取得するステップと、各撮像データに基づいて、各画素について、該撮像データの取得に用いた光に対する生体組織の光吸収を計算するステップと、各画素について、一つ以上の第1撮像データに基づいて計算した生体組織の光吸収の和と一つ以上の第2撮像データに基づいて計算した生体組織の光吸収の和との差分に基づいて第1生体物質と第2生体物質とのモル濃度比を示す指標を計算するステップと、指標に基づいて分布画像を生成するステップと、を含む方法。【選択図】図1
