A drowsiness detection method for detecting drowsiness of a detection target is provided. The method includes a data collecting process including steps of: detecting physiological symptom information of the detection target determining whether the detection target satisfies a predetermined drowsiness threshold condition according to the physiological symptom information when the detection target satisfies the predetermined drowsiness threshold condition, triggering a stimulation event on the detection target determining whether a response event is received from the detection target in response to the stimulation event when the response event is not received, determining whether the stimulation event satisfies a threshold strength condition and recording the physiological symptom information as a corresponding drowsiness detection condition of the detection target when the stimulation event satisfies the threshold strength condition.