A system and method for enhancing diagnosis and treatment including an extended working channel defining an elongated passageway and adapted to extend through the bronchoscope and into a luminal network, an opening operably associated with the extended working channel and in fluid communication with the luminal network, and a medical instrument configured for insertion through the extended working channel, wherein during operation, apposition of tissue on the extended working channel or medical instrument isolates at least a portion of a luminal network in which the extended working channel is inserted.
