The present invention is related to the field of tissue production, regeneration, reconstruction, reinforcement and/or rejuvenation. More precisely, the present invention is related to a method for regenerating specific tissue, preferably mesenchymal tissues like bone, fat, tendon, muscle, cartilage and marrow stroma and hematological tissue (RBC, WBC, platelets, lymphocytes, hematological stem cells (HSC), endothelial progenitor cells (EPC) etc.). The present invention is also related to a method for reconstructing, regenerating, rejuvenating or reinforcing a damaged or diseased tissue in a mammal subject, and preferably a human patient, using a (regenerated) tissue obtained by this method. In one embodiment, the present invention relates to a platelet-rich-plasma (PRP) and/or a coagulation factor, and medicament thereof. In another embodiment, the present invention relates to methods for an in vitro culture and for obtaining non embryonic human stem cells. In another embodiment, the present invention relates to a kit and gel composition.