The present invention teaches anti-Trop-2 monoclonal antibodies with highaffinity and able to recognize differentregions of the Trop-2 molecule, and uses thereof in the treatment anddiagnosis of tumors, such as for example endometrium,breast, head and neck, colon-rectum, stomach, lung, ovary, prostate, pancreas,kidney, cervix and bladder (urothelial) tumors.