An implant for repair of a cartilaginous defect in a subject includes acollagen matrix charged with synovial cells. A method preparing an implant forrepair of a cartilaginous defect in a subject includes obtaining a fluidcontaining synovial cells and charging the synovial cells to the matrix. Adevice for preparing a cell-charged implant includes a first chamber and asecond chamber, the first and second chambers being separated by a membraneand a perforated filter. The membrane is adapted to collect cells from a cell-containing fluid introduced into the first chamber and the perforated filteris adapted to permit passage or diffusion of the fluid through the secondchamber. A method for preparing a cell-charged implant utilizes the device.
