An ultrasound needle positioning system includes an ultrasound probe and a processor. The ultrasound probe is used to capture a plurality of sets of needle insertion images. Each set of needle insertion images includes a plurality of needle insertion images corresponding to a needle body at a predetermined insertion angle. The processor is coupled to the ultrasound probe, and is used to train a first convolutional neural network according to at least one set of needle insertion images in the plurality of sets of needle insertion images to generate needle body positioning information after the needle body is inserted. The needle body positioning information includes a reference position, a length, and/or a width corresponding to the needle body at at least one predetermined insertion angle.
