Líneas celulares derivadas de MDCK adaptadas a un cultivo exento de suero y a un cultivo en suspensión, y procedimiento para la preparación de un virus para una vacuna usando las células
PARK, Yong Wook,PARK YONG WOOK,LEE, Kun Se,LEE KUN SE,LEE, Bong-yong,LEE BONG-YONG,PARK, Mahnhoon,PARK MAHNHOON,KIM, Hun,KIM HUN,KIM, Yun-hee,KIM YUN-HEE,LEE, Su-jeen,LEE SU-JEEN
申请号：
ES11823761
公开号：
ES2695044T3
申请日:
2011.09.06
申请国别(地区):
ES
年份:
2018
代理人:
摘要：
The invention discloses a kind of Martins to reach the cell line than the source dog kidney passage cell (MDCK). The mdck cell that the cell line in the sources MDCK is ATCC CCL-34 from the number of preservation. The cell line in the sources MDCK can pass through free serum culture and the culture preparation that suspends. Preferably, the cell line in the sources MDCK has low oncogenicity or without oncogenicity. It is preferred that the cell line in the sources MDCK is selected from MDCK Sky1023, MDCK Sky10234 and MDCK Sky3851. The method for being further disclosed that the cultural method for cultivating the cell in the sources MDCK and preparing vaccine virus using the cell in the sources MDCK.
