PROCÉDÉ DE PRODUCTION DE MACROBILLES CONTENANT DES NANOPARTICULES RÉSISTANT AUX ACIDES ET UTILISATION DE MACROBILLES POUR DÉCLENCHER UNE RÉPONSE IMMUNITAIRE
The present invention relates to methods and means for the production of alginate/chitosan acid-resistant macrobeads comprising alginate/chitosan nanoparticles, wherein the nanoparticles comprise a bioactive agent, wherein the bioactive agent is associated via physicochemical interaction with a polymer. The invention further relates to nanoparticles and/or macrobeads obtained by methods of the invention and such nanoparticles and/or macrobeads for use in eliciting an immune response in an animal. Also encompassed by the invention is the use of a porcine epidemic diarrhea virus (PEDV) antigen as defined herein for producing a nanoparticle or acid-resistant macrobeads and a vaccine comprising such an antigen.
