Obshchestvo s ogranichennoj otvetstvennostyu "Farmag"
发明人:
Tishin Aleksandr Mettalinovich,Тишин Александр Метталинович,Pyatakov Aleksandr Pavlovich,Пятаков Александр Павлович,Shtil Aleksandr Albertovich,Штиль Александр Альбертович,Gunko Yurij Kuzmich,Гунько Ю
申请号：
RU2016112370
公开号：
RU0002633918C2
申请日:
2016.04.01
申请国别(地区):
RU
年份:
2017
代理人:
摘要：
FIELD: medicine.SUBSTANCE: application of Zn-substituted magnetic nanoparticles based on manganese ferrite of the formula ZnxMn1-xFe2O4 (x=0-0.9), with average size of less than 40 nm, the Curie temperature ranges from 39 to 550°C, and the coercive force is 5 to 250 Oe. A pharmaceutical composition comprising these Zn-substituted magnetic nanoparticles and a pharmaceutically acceptable carrier is also provided. A method for malignant neoplasms treatment with magnetic hyperthermia, including the following steps (i) and (ii), is also proposed. Step (i) includes magnetic nanoparticles delivery to the tumour region in an effective concentration, selected from the group of magnetic metal oxides including Zn-substituted magnetic nanoparticles based on manganese ferrite of the formula ZnxMn1-xFe2O4 (x=0-0.9), with average size of less than 40 nm, the Curie temperature ranges from 39 to 550°C, and the average value of coercive force is 5 to 250 Oe. The step includes (ii) tumour region exposure to an alternating current (AC) magnetic field with an amplitude of 14 to 300 Oe and a frequency of 80 to 1000 kHz, where the exposure time of the said magnetic field at a temperature of 38 to 51°C is between 15 and 60 minutes. In addition, a method for experimental tumour cells damage by magnetic hyperthermia is proposed, comprising the following steps (i), (ii), (iii). At that, step (i) includes provision of magnetic nanoparticles selected from the group of magnetic metal oxides including Zn-substituted magnetic nanoparticles based on manganese ferrite of the formula ZnxMn1-xFe2O4 (x=0-0.9), with average size of less than 40 nm, the Curie temperature ranges from 39 to 550°C, and the average value of coercive force is from 5 to 250 Oe. Step (ii) includes addition of the said magnetic nanoparticles to the cell culture. Step (iii) comprises exposure of the said cell culture to a magnetic field of PT with an amplitude of 14 to 300°C and frequency of 80 to 1000 kHz, where the exposure tim
