The present invention relates to chimeric antigen receptors (CARs) specific to Intercellular adhesion molecule-1 (ICAM-1) comprising I domain of the aL subunit of human lymphocyte function-associated antigen 1 (LFA-1 ). The invention particularly relates to CARs comprising human I domains having different affinities (1 mM to 1 nM Kd) to ICAM-1. CAR-T cells comprising human I domain having a low affinity (1 to 200 uM Kd) to ICAM-1 can avoid targeting healthy tissues with basal ICAM-1 expression while simultaneously exhibiting increased potency and long-term efficacy against tumor tissues with high ICAM-1 expression. The present invention also relates to an adoptive cell therapy method for treating cancer by administering the CAR-T cells comprising human I domain to a subject suffering from cancer, whereby the CAR-T cells bind to the cancer cells overexpressing ICAM-1 and kill the cancer cells.
