コレステロールペレット固形製剤を用いたLHRHaの埋め込み投与によるマハタ親魚の成熟・排卵誘導法

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专利权人:

長崎県

发明人:

中田 久,征矢野 清,松山 倫也,宮木 廉夫

申请号：

JP2006084326

公开号：

JP4899569B2

申请日:

2006.03.25

申请国别(地区):

JP

年份:

2012

代理人:

摘要：

PROBLEM TO BE SOLVED: To provide a stable and efficient spawning method for sevenband grouper by using LHRHa as a maturation- and ovulation-inducing hormone agent, utilizing the technique for increasing its persistence and enabling the sustained-release dosage.

SOLUTION: A cholesterol pellet is used as a solid preparation that enables the sustained-release dosage of a synthetic luteinizing hormone-releasing hormone (LHRHa : des-Gly10, [D-Ala6]-LHRH ethyl amide), the dosage of the LHRHa is set to 20 to 100 &mug/kg BW and the adult groupers are selected from adult groupers cultured under artificial environment, using an individual egg selected from the eggs having a diameter of 420 to 550 &mum in the ovary. The eggs collected after the hormone treatment are artificially fertilized, thus the fertilized eggs of good quality can stably and efficiently obtained from the adult groupers.

COPYRIGHT: (C)2008,JPO&INPIT

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