A puncturing device for extracting organic samples, and which includes a puncturing cannula ending in a needle tip, the lumen of the puncturing cannula being designed as a suction channel, and a flushing line for supplying a flushing agent. Behind the needle tip of the puncturing cannula a single-lumen puncturing area is provided, which is followed by a double-lumen area departing from a connecting site at which the flushing line is fluid-tightly attached to the puncturing cannula. At a distal end of the flushing line is provided at least one transfer opening into the suction channel of the puncturing cannula.