AUERBACH, Wojtek,FRENDEWEY, David,DROGUETT, Gustavo,GAGLIARDI, Anthony,KUNO, Junko,VALENZUELA, David M.
申请号：
ES15729724
公开号：
ES2784754T3
申请日:
2015.06.05
申请国别(地区):
ES
年份:
2020
代理人:
摘要：
Methods and compositions are provided for modifying one or more target loci in a cell. Such methods comprise providing a cell comprising a first polynucleotide encoding a first selection marker operably linked to a first promoter active in the cell, wherein the first polynucleotide further comprises a first recognition site for a first nuclease agent. A first nuclease agent is introduced into a cell, wherein the first nuclease agent induces a nick or double-strand break at the first recognition site. Further introduced into the cell is a first targeting vector comprising a first insert polynucleotide flanked by a first and a second homology arm that correspond to a first and a second target site located in sufficient proximity to the first recognition site. At least one cell is then identified comprising in its genome the first insert polynucleotide integrated at the target locus.
