California Institute of Technology;The Regents of the University of California
发明人:
Chen, Irvin S.Y.,Lois-Caballe, Carlos,Qin, Xiao-Feng,An, Dong Sung,Baltimore, David
申请号：
AU2008222663
公开号：
AU2008222663B2
申请日:
2008.03.07
申请国别(地区):
AU
年份:
2013
代理人:
摘要：
In one aspect, the invention provides methods and compositions for the expressionof small RNA molecules within a cell using a retroviral vector (Fig. IA). Smallinterfering RNA (siRNA) can be expressed using the methods of the invention withina cell. In a further aspect, the invention provides methods for producing siRNAencoding lentivirus where the siRNA activity may interfere with the lentivirallife cycle. In yet a further aspect, the invention provides methods for expressionof a small RNA molecule within a cell, such as an siRNA capable of downregulatingCCR5, wherein expression of the small RNA molecule is relatively non-cytotoxicto the cell. The invention also includes small RNA molecules, such as an siRNAcapable of downregulating CCR5, that are relatively non-cytotoxic to cells.
