The present invention relates to the technical field of antibody engineering.Providedare a full human anti-PD-1 monoclonal antibody, a preparation method therefor,anduses thereof. Anti-PD-1 monoclonal antibodies are obtained from a fullysyntheticantibody library by means of screening, and then light-chain CDR1-3-region andheavy-chain CDR1-3-region mutation construction libraries of the obtainedantibodyare screened in sequence by means of an affinity maturation technology toobtain ahigh-affinity anti-PD-1 antibody. The antibody can be used for treatingtumors,inflammations, and autoimmune diseases.
