An infant pacifier sterilization assembly includes a housing that has an aperture extending through a top of the housing. An antiseptic compound is contained within the housing. The antiseptic compound is nontoxic. A valve is positioned within the aperture. The valve insertably receives a nipple on a pacifier so the nipple is submerged in the antiseptic compound. The valve is a one-way valve so the antiseptic compound is retained in the housing.