Provided is a spiral soft tissue biopsy needle, comprising a puncture needle rod (1), a sampling needle rod (2), and a hand shank (3) for fixing the puncture needle rod (1) and the sampling needle rod (2), wherein the sampling needle rod (2) comprises a spiral needle rod (21) for fixing the sampling site and a cutting needle rod (22) for separating the sample from the sampling site, the spiral needle rod (21) has a hollow structure, the front end of the spiral needle rod (21) is provided with a spring spiral tube (211), and the front end of the cutting needle rod (22) is provided with a cutting edge (221). The spiral soft tissue biopsy needle of the present invention can improve the success rate of one-time sampling and achieve a multi-point sampling in different positions.