Federalnoe gosudarstvennoe byudzhetnoe obrazovatelnoe uchrezhdenie vysshego obrazovaniya "Kubanskij gosudarstvennyj agrarnyj universitet imeni I.T. Trubilina"
发明人:
Terekhov Vladimir Ivanovich,Терехов Владимир Иванович,Tishchenko Aleksandr Sergeevich,Тищенко Александр Сергеевич
申请号：
RU2017118742
公开号：
RU0002650628C1
申请日:
2017.05.29
申请国别(地区):
RU
年份:
2018
代理人:
摘要：
FIELD: biotechnology.SUBSTANCE: invention is a method of producing a vaccine associated with colibacillosis, streptococcosis and enterococcal infection of calves and piglets, including the production of antigens by the isolation from diseased organs of calves and piglets of epizootic strains of Escherichia coli producing exotoxins, hemolytic strains Streptococcus bovis and Enterococcus faecalis, cultivation of which is carried out separately on a nutrient broth, while the cultures of Escherichia coli are grown for 7 days, and cultures Streptococcus bovis and Enterococcus faecalis for 24 hours to a concentration of 5–6 billion microbial cells per ml, after which they are inactivated with formalin to a final concentration of 0.3–0.4 % for 14 days, where after formalin inactivation of the antigens, sterilizing filtration of Escherichia coli broth culture is carried out to obtain a cell-free culture medium containing toxins of Escherichia coli, which is mixed with culture media containing cells of hemolytic strains Streptococcus bovis and Enterococcus faecalis, at the following components ratio, mass%: culture medium containing cells of hemolytic strains Streptococcus bovis – 26.0–30.0 culture medium containing cells of hemolytic strains Enterococcus faecalis – 26.0–30.0 cell-free culture medium containing toxins of Escherichia coli is the rest.EFFECT: invention allows to increase effectiveness of specific prevention of colibacillosis, streptococcosis and enterococcal infection in calves and piglets, to increase the safety and harmlessness of the vaccine.1 cl, 2 tbl, 7 exИзобретение относится к биотехнологии. Изобретение представляет собой способ получения вакцины ассоциированной против колибактериоза, стрептококкоза и энтерококковой инфекции телят и поросят, включающий получение антигенов путем выделения из пораженных органов телят и поросят эпизоотических штаммов Escherichia coli, продуцирующих экзотоксины, гемолитических штаммов Streptococcus bovis и Enterococcus fae
