Provided is a non-invasive biological substance measuring device capable of stably and highly accurately measuring the amount of a biological substance. The biological substance measuring apparatus 80 has a first light source 32 that emits first light, a front surface and a back surface, and the ATR prism 20 in which the first light incident from one end passes through the inside and is emitted from the other end. And a hyperbolic metamaterial layer 90 that has a front surface and a back surface and is arranged so that the back surface is in contact with the front surface of the ATR prism 20, and a first light detection that detects the first light emitted from the ATR prism 20. And a container 30 for measuring the amount of the biological substance in the living body from the detected first light.生体物質の量を安定的にかつ高精度に測定できる非侵襲型の生体物質測定装置を提供する。生体物質測定装置８０は、第１の光を放射する第１の光源３２と、表面と裏面を有し、一端から入射した第１の光が内部を透過して他端から出射するＡＴＲプリズム２０と、表面と裏面を有し、ＡＴＲプリズム２０の表面上に裏面が接するように配置されたハイパボリックメタマテリアル層９０と、ＡＴＲプリズム２０から出射された第１の光を検出する第１の光検出器３０と、を備え、検出された第１の光から生体中の生体物質の量を測定する。
