The invention relates to a whole blood filtering device, which comprises a first filtering part, a second filtering part disposed on the first filtering part, and a microfluidic device, in which the first filtering part comprises a plurality of filtering membranes with micropores distributed along a plane, while the second filtering part comprises a plurality of beads, and the size of the beads near an output end is larger than that near an input end of the second filtering part. By the downward pressure of blood and the suction of the microfluidic device, blood cells can therefore be separated and detected through the first and the second filtering part in a short time.本發明關於一種全血過濾裝置，包括一第一過濾部、一與該第一過濾部連接的第二過濾部、以及一微流道部，其中，該第一過濾部包括複數個各具有複數個沿一平面分布的微孔的過濾膜，而該第二過濾部包括複數個微珠，且於該第二過濾部之輸出端的微珠尺寸大於輸入端的微珠尺寸，據此，藉由血液往下的壓力以及該微流道部的吸力，可在短時間內使血液經該第一過濾部及該第二過濾部得以進行分離而檢測。
