Problem to be solved: the present invention provides a novel gene, WMS, which provides wheat male sterility, its anther specific expression promoter and their use.In China, the well-known gene MS2 which causes dominant male sterility has been widely applied to the circulation selection of wheat. An anther specific transcriptome of a pair of MS2 homologue \"Lumai 15\" and \"Lumai 15 + MS2\" was carried out by the RNA SEQ method. As the result, the WMS gene which showed the anther peculiar expression in the stage of meiosis was identified only in the wheat which had the MS2 gene.Regulation of WMS can alter the male fertility of plants. The promoter of WMS has an anther specific activity. Accordingly, the present invention can be used to achieve anther specific gene expression, expression of male sterility in various plant species, establishment of cyclic selection of various plant species, and assistance of hybrid seed production.Diagram【課題】本発明は、コムギ雄性不稔性を付与する新規な遺伝子WMS、その葯特異的発現プロモーターおよびそれらの使用を提供する。【解決手段】中国では、優性雄性不稔を引き起こすよく知られた遺伝子Ms2がコムギの循環選抜に広く応用されてきた。RNA-seq法を実施して1対のMs2同質遺伝子系統「Lumai 15」および「Lumai 15+Ms2」の葯特異的トランスクリプトームを明らかにした。その結果、Ms2遺伝子を保有するコムギにのみ減数分裂の初期段階で葯特異的発現を示すWMS遺伝子が同定された。WMSの調節によって植物の雄性稔性を変化させ得る。WMSのプロモーターは葯特異的活性を備えていることがわかった。したがって、本発明を葯特異的遺伝子発現の達成、様々な植物種の雄性不稔性の発現、様々な植物種の循環選抜の確立およびハイブリッド種子生産の補助に用い得る。【選択図】図1
