Genetic construct, recombinant vector, method of increasing the concentration of pck and / or ppdk in a leaf of a plant, method of decreasing the concentration of nitrogen in the leaves of a plant, method of increasing the growth rate of a plant, cell, transgenic plant, plant propagation product, production method of a transgenic plant that remobilizes nitrogen at a higher rate, production method of a transgenic plant presenting a higher growth rate, leaf collected containing a lower nitrogen level, smoking article .
JULIAN MICHAEL HIBBERD,LUCY ELISABETH TAYLOR,ANNA ELIZABETH LEISS
申请号：
BRPI1007835
公开号：
BRPI1007835A2
申请日:
2010.02.25
申请国别(地区):
BR
年份:
2015
代理人:
摘要：
The invention provides genetic constructs which comprise a senescence-specific promoter operably linked to at least one coding sequence, which encodes at least one polypeptide having phosphoenolpyruvate carboxykinase (PCK) activity and/or pyruvate orthophosphate dikinase (PPDK) activity. The constructs have the ability to cause, in transgenic plants, remobilisation of nitrogen during leaf senescence, such that nitrogen can be transported from the leaves to other regions of the plants. The invention provides plant cells and plants transformed with such constructs, methods of producing transgenic plants, and methods of increasing the rate of nitrogen remobilisation and growth rate in senescent plants. The invention also provides harvested plant leaves, such as tobacco leaves, that have been transformed with the genetic constructs, and to smoking articles comprising such harvested plant leaves.
