An OCT microprobe applied to endoscopic high-resolution optical coherence tomography, an imaging system, and a use method, which relate to the technical field of medical instruments. By using a lens assembly, the OCT microprobe changes a working distance of the OCT microprobe, and increases a light aperture of a grin lens (4), thereby improving a numerical aperture and horizontal resolution of the OCT microprobe. The present invention can resolve a problem that in an existing OCT system, an OCT microprobe has low horizontal resolution and an image obtained through scanning changes easily. Therefore, OCT endoscopic scanning imaging with the OCT microprobe is applicable to blood vessels, digestive tracts, respiratory tracts or narrow space of human tissue.
