A system for detection of bilirubin content in neonates skin includes at least one camera configured to capture at least one image of neonates skin and a reference image, a display system, a communication interface and a control unit configured to control functioning of other elements of the system. The control unit is further configured to detect bilirubin level by comparing image of neonates skin and reference image. Furthermore, the control unit is configured to transfer said image of neonates skin to at least one of communication interface and display system. The reference image is a single image acquired from any one of the dermal zones or multiple images from more than one dermal zones of neonate under observation.